What are critical in plant tissue culture..????

A suitable culture medium with growth Regulators/Extracts

Culture Room with controlled conditions for proper growth.

Sterile/Aseptic conditions to transfer the Explants in the culture medis

Sterile Explants

Proper instruments/Chemicals/Reagents etc.

• Appropriate tissue 
• A suitable growth medium 
• sterile conditions 
• Growth regulators 
• Frequent subculturing

1. A suitable growth medium,2. Aseptic condition,3. Growth regulators,4. Sterilization5. Culturing (Micropropogation) plant tissue.