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Why temperature is important in gas chromatography?

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تم إضافة السؤال من قبل Qaisar Abbas
تاريخ النشر: 2014/05/17
عبد السلام هلال
من قبل عبد السلام هلال , TECHNIQUE EDUCATION , EDUCATION

tempreture have  a direct affect in gaz chromatography

if we use the relation  P V=n R T     R  is constante

Volume and Temprature is proportional to the Pressure

so if the temprature increase the pressure or /and the volume increase

when we say volume we say the teh gaz flow  then the atomic matter adherance

so the temprature have a direct affect in the fiablty of chromatography

Mohamed Khedr
من قبل Mohamed Khedr , Researcher , Helmholtz Center Munich

When it comes to GC it's all about temperature:

First of all, injection temperature must be selected to homogenously evaporate the sample) especially if you're using Headspace injector!), selecting lower temperature would result in very weak signals and selecting higher temperature would result in the decomposition of components or the explosion of the sample vial! That's why you've to select the exactly suitable temperature, not a bit higher or lower! 

 

Secondly, the column temperature program is essential for the resoultion of the components peaks. As explained by Mr. Helal, the higher the temperature the higher the pressure will get causing the peaks to get closer and eventually overlap!

 

Thirdly, for the detector's temperature (especially if you are using a temperature depndant technique like flame ionization). The temperature must be sufficient to detect readable signal or else, you'll have no clue of the separation's occerence no matter how perfect your method already is!

GC

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