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How many type of media we use in microbiology lab ??

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Question added by mohamed akasha ali mahmoud , Laboratory technologist , Al Borg laboratory
Date Posted: 2014/07/09
Dr.AHMAD EBRAHIM,
by Dr.AHMAD EBRAHIM, , ‘Veterinary Microbiologist’ , Ministry of Municipality&Environment

ROUTINE LABORATORY MEDIA are classified into six types: (1) Basal media, (2) Enriched media, (3) Selective media, (4) Indicator media, (5) Transport media, and (6) Storage media.

1.   BASAL MEDIA. Basal media are those that may be used for growth (culture) of bacteria that do not need enrichment of the media. Examples: Nutrient broth, nutrient agar and peptone water. Staphylococcus and Enterobacteriaceae grow in these media.2.     ENRICHED MEDIA. The media are enriched usually by adding blood, serum or egg. Examples: Enriched media are blood agar and Lowenstein-Jensen media. Streptococci grow in blood agar media.3.   SELECTIVE MEDIA. These media favour the growth of a particular bacterium by inhibiting the growth of undesired bacteria and allowing growth of desirable bacteria. Examples: MacConkey agar, Lowenstein-Jensen media, tellurite media (Tellurite inhibits the growth of most of the throat organisms except diphtheria bacilli). Antibiotic may be added to a medium for inhibition.4.     INDICATOR (DIFFERENTIAL) MEDIA. An indicator is included in the medium. A particular organism causes change in the indicator, e.g. blood, neutral red, tellurite. Examples: Blood agar and MacConkey agar are indicator media.5.   TRANSPORT MEDIA. These media are used when specie-men cannot be cultured soon after collection. Examples: Cary-Blair medium, Amies medium, Stuart medium.6.   STORAGE MEDIA. Media used for storing the bacteria for a long period of time. Examples: Egg saline medium, chalk cooked meat broth.

Arunkumar Sreekumaran
by Arunkumar Sreekumaran , MEDICAL LABORATORY TECHNOLOGIST , ABUDHABI HEALTH SERVICES

Thanks for the questions.

 

In Microbiology Laboratory3 types are routinely used

1.Liquid Media (Broths)

2.Semi solid Media

3.Agar Plates.

 

Depends on the nature of disease,clinical specimen type and transportaion,different types of Media are applied

 

Example

 stool Sample we can incubate it in Selenite F Broth and Mac Conkey agar.After12 hrs of Incubation we can again subclutre from selenite F Broth  to Macconkey agar or Salmonella Shigella agar or any selective media.

Urine

Preferred Media are Blood agar,Mac Conkey agar and CLED agar.

CSF

Blood agar,Macconkey agar Chocolate Agar.

 

Swabs

Blood Agar,Chocolate Agar,Macconkey Agar,

 

Cavity fluids

Robertson Cooked meat Medium for Anaerobiasis. BA,CA

 

Fungal Specimens

Sabrouds Dextros agar

Eugene ode
by Eugene ode , Microbiology Specialist , Ministry Of Education - Saudi Arabia

We used three types of media in microbiology

Ahmed Elmodather Farghly Esmail elmodather
by Ahmed Elmodather Farghly Esmail elmodather , Microbiology Laboratory Manager , Hochster pharmaceutical industry

There are Different types of media we can use in Microbiology lab according to your field and your work.there are selective medium to isolate specific Microorganism as Baird Parker  agar media to isolate Staph aureus  Etc....

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