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- So this chromatographic technique is the first kind of HPLC ever used.
- It uses the solubility of the analytes (molecules to be separated) in two immiscible (non-mixing) liquids. One of the liquids is used as the eluant, the other is used as the stationary phase.
- The analytes partition, or move back and forth, between the eluant and the stationary phase at certain rates, depending on the characteristics of each analyte.
- Therefore, certain analytes will elute first because they solvate in the eluant at a faster rate, while other analytes will elute last, due to their higher solubility in the stationary phase.
Partition Chromatography mean by " solute mixing with mobile phase and moving through the stationary phase at the time the solute partition between mobile phase and stationary phase. It is called Normal Phase chromatography.
