In some cases microbial count is less in initial suspension when compared to the further dilution, why?

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In manual white blood cell count using haemocytometer the dilution used is:
Posted by:
mohammad ali
14-November-2014

Pharmaceutical Research

Question added by shara Daram , Deputy Manager , Lucid Laboratory Pvt Ltd
Date Posted: 2014/09/19

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by shara Daram , Deputy Manager , Lucid Laboratory Pvt Ltd

As the inhibitory concentration is more less colonies are observed in initial suspension, as when dilution increases it get decreased and the colonies increase.

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In some cases microbial count is less in initial suspension when compared to the further dilution, why?

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