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What are critical in plant tissue culture..????

Plant Tissue Culture
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Question added by Sabeetha Senevirathne , Research Assistant , Field Crops Research and Development Institute -DOA
Date Posted: 2014/10/06
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MUHAMMAD ANWAR MALLICK
by MUHAMMAD ANWAR MALLICK , Director, Biotechnology & Head, Department of Botany , VINOBA BHAVE UNIVERSITY,HAZARIBAGH

A suitable culture medium with growth Regulators/Extracts

Culture Room with controlled conditions for proper growth.

Sterile/Aseptic conditions to transfer the Explants in the culture medis

Sterile Explants

Proper instruments/Chemicals/Reagents etc.

Abdullah Al Hinidi Al Hinidi
by Abdullah Al Hinidi Al Hinidi , COMMERCIAL OPERATOR , RENFE

  • Appropriate tissue 
  • A suitable growth medium 
  • sterile conditions 
  • Growth regulators 
  • Frequent subculturing

Mohd Fahad Parvez
by Mohd Fahad Parvez , QA/QC Executive , Allanasons Pvt Ltd

1. A suitable growth medium,2. Aseptic condition,3. Growth regulators,4. Sterilization5. Culturing (Micropropogation) plant tissue.

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