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For blood culture the best anticoagulant is:

<p><strong><span>a- Citrate</span></strong></p> <p><strong><span>b-Oxalate</span></strong></p> <p><strong><span><span>c- Sodium polyanesthol sulphonate(SPS)</span></span></strong></p> <p><strong><span>d- Heparin</span></strong></p> <p> </p> <p><span><strong>e- EDITA</strong></span></p>

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Question added by mohammad ali , رئيس قسم المختبرات , مؤسسة ماحد التطوير
Date Posted: 2014/11/21
Mozfar Idrees
by Mozfar Idrees , Medical Laboratory Director , Asia Polyclinic

SPS

Nabil Hijazi
by Nabil Hijazi , Director , Biocell Lab

Sodium polyanesthol sulphonate(SPS)

muhammad rababah
by muhammad rababah , jordan , royal medical

C sps .............................................

zubair farooq
by zubair farooq , supervisor of clinical lab , aznostics

a- Citrate b-Oxalate c- Sodium polyanesthol sulphonate(SPS) d- Heparin   e- EDITA

 

Ans: Tryptic soya broth 

haneen alwahsh
by haneen alwahsh , medical technologist , biolab

C

Mohamed  shaheen
by Mohamed shaheen , مدرس كلينيكال باثولوجي , كلية الطب جامعة الأزهر

Sodium polyanetholesulfonate (SPS), an anticoagulant which inhibits the antimicrobial systems of blood, is used widely in blood culture media. The addition of SPS to experimental blood cultures inoculated with small numbers of a variety of organisms caused a striking increase in recovery of these organisms. Sodium fluoride also increased the incidence of positive blood cultures with some organisms. SPS completely inhibited serum antibacterial activity and serum-dependent phagocytosis (and killing) by isolated leukocytes at a concentration usually employed in blood culture media. SPS also stimulated both glucose C-1 oxidation in resting leukocytes and formate oxidation in both resting and phagocytosing leukocytes in serum-free systems. These in vitro studies support the concept that SPS is a useful additive to blood culture media and further elaborate on the mechanism of its inhibition of the microbicidal activity of blood.

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