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A plasma sample sent to the lab for PT testing has been stored for 25 hours at 4°C. The PT result is shortened. What is the most probable cause?

<p>A. Factor VII deficiency</p> <p>B. Activation of factor VII due to exposure to coldtemperature</p> <p>C. Lupus inhibitor</p> <p>D. Factor X inhibitor</p>

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Question added by Mozfar Idrees , Medical Laboratory Director , Asia Polyclinic
Date Posted: 2014/12/09
bahjat alabou houseen
by bahjat alabou houseen , clinical hematologist , KING ABDULAZIZ SPECIALIZED HOSPITAL IN TAIF

The anticoagulated blood should be mixed gently by inversion three or four times, sent to the laboratory in an expeditious manner, and tested within two hours if kept at room temperature (22 to24°C) or within four hours if kept cold (2 to4°C), so that the entire interval between phlebotomy and testing does not exceed24 hours. Prolonged incubation of blood at ambient temperatures for >24 hours results in significantly decreased activity of factors V, VIII, and protein S.

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