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206 total bones, and femur or thigh bone us longest bone in the body
To extract the RNA from a tissue or a cell suspension, it is imperative to inhibit traces of RNase.
Homogenization in the Potter tube is done in a Tris-EDTA buffer in the presence of 4M guanidinium thiocyanate and SDS. Cellular debris is pelleted by 10 min at 10 ° C.
The supernatant containing the nucleic acids is deposited on a density gradient tube comprising at the bottom of a dense CsCl solution topped 5.7M of an intermediate layer of 2.4 M. This CsCl gradient was ultracentrifuged for 24 hours at 30000 revolutions / min at room temperature.
We can then collect a high molecular weight RNA pellet will be subjected to extraction with phenol and chloroform.
206 bones are present and logest bone is femur
THE LONGEST IS THE FEMUR BONE
THE TOTAL NUMBER OF THE HUMEN BODY BONES IS 206
There are 206 bones in the human body and femur is the longest bone of the human body