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Pixels (px):
px are browser dependent. It is the absolute size that you would see on your screen.
em:
Em are sort of like percentages. Ems is referring to the base text size. The value of 1 emmeans the same thing as a value of 100 percent. But you can also say it in the opposite way: A percentage value is just an em multiplied by 100.
px stands for pixels, generally use to define dimensions for fonts, pictures, tags, Divs, etc in absolute terms
em is used for sizing fonts in multiple values of the set font size. If font size is set to 12px, it will be 1 em. 2em will be twice the size of the set font i.e 24 px
percentages are generally used for responsive webdesigns to accommodate the design/fonts as per the screen size.
