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There is a method to clone a gene without the use of restriction enzymes. Anyone here knows the strategy? It's a primitive technique (hint)!

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Question added by Mohammed Jaseem , Academics Support Specialist - Preceptor , Biotecnika
Date Posted: 2016/07/08
Haifa Al qudaihy
by Haifa Al qudaihy , Deputy Manager PGD Laboratory , Saad Specialist Hospital

There are different methods like:

Toposiomerase technology,Sequence and ligase independent cloning, 

pour cloner un gêne sans utilise des enzymes de restriction , on peut se référer a une méthode  simple , nommé la technologie des topoisomerases ,  :  

       La technologie utilisé l'activité biologique inhérente de la topoisomérase ADN I. Le rôle biologique de la topoisomérase est de cliver et de rejoindre les terminaisons de  l'ADN superenroulé  pour faciliter    la réplication. topoisomérase I du virus de la vaccine reconnaît spécifiquement la séquence d'ADN 5 '(C / T) CCTT-3'. Lors de la réplication, l'enzyme ADN digère spécifiquement cette séquence, l'ADN se déroule et relié  à nouveau au groupe phosphate 3 'de la base de la thymidine. 

Deleted user
by Deleted user

Gene can be cloned using PCR (polymerase chain reaction) technique. In this technique copies of fragmented DNA is made using PCR. Those fragmented DNAs are used for making gene library. Further analysis can be carried out with the library created. But this technique requires prior knowledge of nucleotide sequence to be copied. Moreover this technique is very sensitive to small amount of contamination. 

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