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The different stationary phases as in reversed and normal phase chromatography?

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Question added by Victoria Anderson
Date Posted: 2016/09/25
Ankita Gambhir
by Ankita Gambhir , Lab Specialist , Scientific Diamond Company

Typical stationary phases for normal-phase chromatography are silica or organic moieties with cyano and amino functional groups.

For reversed phase, alkyl hydrocarbons are the preferred stationary phase; octadecyl (C18) is the most common stationary phase, but octyl (C8) and butyl (C4) are also used in some applications. 

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