What is the difference between normal and reverse phase chromatography?

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What is the difference between normal and reverse phase chromatography?
Posted by:
Laura M
12-January-2016

Question added by safia islahi
Date Posted: 2016/10/09

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1 Answers

by Ankita Gambhir , Lab Specialist , Scientific Diamond Company

Normal Phase Chromatography

Stationary Phase : Polar

Mobile Phase: Non-Polar

order of elution- Non-Polar to Polar

Reverse Phase Chromatography:

Stationary Phase : Non-Polar

Mobile Phase: Polar

order of elution- Polar to Non-Polar

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