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Based on Beer lambert s law for the relationship between absorbance and the substrate concentration for quantitative analysis
UV visible spectroscopy owrking based on The Beer-Lambert law is the linear relationship between absorbance and concentration of an absorbing species.
Absorption of UV or visible light. Conjugated systems like a benzene ring absorb UV light efficiently
The basic principle of UV visivle spectroscopy is that the absorption of light of a solution is dirrectly proportional to the concentration of that solution.
The amount of light absorbed by a solution is directly proportional to the concentration of the solution.
The Basic Principleof UV-Vis Spectrophotometer is based on the beer's Lambert low. In which a beam of light is passed through the solution absorb the monochromatic radiation of solution by a substance.
it's a moleculaire spectroscopie technic in UV and Visible wevelenghth range from about 190 to 1100nm )used for qualitiative ( scan and wevlegth methode for spetre determination and identification of molecule )and quantitative methode when coumpound are identified and need to be quantifyed ( calibration and mesure )
the principle is based on the Beer-Lambert law which states that when a beam of monochromatic light is passed through a solution of an absorbing substance the rate of decrease pf intensity of radiation with thickness of the absorbing solutin is proportional to the incident radiation as well as the concentration of the solution
A=log(Lo/L)=EcL from the expression of Beer- Lambert law
UV/Visible Spectroscopy is based on the principle A=epsilon.ct which is comes from Lambert and beers law of absorption(A) . Absorbed light is directky proportional to (species present in the test solution) concentration and thickness of the medium .
UV/Visible Spectroscopy works on Lambert beer law of absorption . Absorbed light is directky proportional to concentration of the species present in the test solution.
UV spectroscopy is type of absorption spectroscopy in which light of ultra-violet region (200-400 nm.) is absorbed by the molecule. Absorption of the ultra-violet radiations results in the excitation of the electrons from the ground state to higher energy state. The energy of the ultra-violet radiation that are absorbed is equal to the energy difference between the ground state and higher energy states (deltaE = hf)
