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we can diagnosis with to method are HB ELECTRPHOSIS and RBC morphology
You doubt the SCD if the hemoglobin level is low and the number of reticulocytes is high. This indicates that hemolysis occurs. Then you need to make a blood smear and if you identify sickle-shaped cells under a microscope all you have to do is to perform hemoglobin electrophoresis test to confirm types of abnormal hemoglobin form present.
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