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As an assembly language developer for years:
I can say DEFINITELY YES
till the moment there are some parts in software that must be written in assembly language like parts of drivers
also if you want to use some special instruction set provided by the processor , which the compiler know little about for example: Using SIMD instructions to optimize performance
in Intel x86 processors these instructions are named: MMX, SSE1, SSE2,SSE3,SSE4,AVX
and on ARM architecture the latest sets are : NEON, and VFP3