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Mention the difference between DNA and RNA gel electrophoresis ?(handling according structure)

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Question added by mohamed sharaf , Assistant Lecturer of Biochemistry , Faculty of Sciences Port Said University
Date Posted: 2014/06/10
Amanpreet Kaur
by Amanpreet Kaur , Senior research fellow , University Grants Commission, New Delhi

In gel electrophoresis, molecules separate on the basis of charge, molecular weight and size. Thus, if we load DNA and RNA samples in the agarose gel for electrophoresis, different type of bands will be visible on EtBr staining. In well with DNA sample, a single band will b visible, provided DNA is not degraded. In case of degradation, a smear will be seen. In well with RNA sample, two bands will be visible, at about 500-600 bp. These bands are of RNA subunits 28s and 18s.

Bilal Alkhizzi
by Bilal Alkhizzi , Administrative Manager , Itqan Medical Center

If I'm understanding your question correctly, make sure to add an RNAse inhibitor to your RNA sample.

Deleted user
by Deleted user

Iam not sure what you trying to ask,

I have performed gel electrophoresis many times. However, i used it mainly for DNA identification by the usage of restriction enzymes. I used to get RNA contamination sometimes, but it did not migrate as DNA. RNA looked very bright in colour and they remained in the wells.

to my understanding DNA and RNA are both macromolecules, so iam not sure what you mean by ''handling".

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