Trending Ion Channels Discussions

How the HPLC colum and ion chromatgraphic colum is re genrate‎

 

Answer added by  Aavian Hapanyengwi, qc chemist, Kiarah Chemicals CC
7 years ago

You regenerate HPLC columns by flushing  it with the following volumes of solvent. 20 column volumes of water/acetonitrile (95:5 v/v) 20 column volumes of acetonitrile 5 ... See More

 

Answer added by  Zisan Ahamad, Project Trainee, CSIR-CDRI
7 years ago

by multiple washing with mobile phase they can be regenerate

 

Answer added by  Faraz ASKARI ABIDI, Quality control officer, Pfizer Pakistan
7 years ago

HPLC columns is regenerate by washing washing with purified water and preserve with methanol or Acetonytrle 50% depend open the type of mobile phase we are using

 

Answer added by  Virendra Vikhare, chemist, Rezilabs for Industrial Services / SAECO
7 years ago

To clean and regenerate a reversed phase column flush it with the following volumes of solvent.  water/acetonitrile (95:5 v/v)  acetonitrile  isopropanol hexane  isopropa ... See More

 

Answer added by  ABDULRAHIM MAHEBOOB MOMIN MOMIN, SR OFFICER, WOCKHARDT BIOTECH PARK
8 years ago

BY PASSING 1 M NACL SOLUTION THROUGH CHROMATOGRAPHY COLUMN.

 

Answer added by  KRISHNAMOORTHI THANGAVEL, Research Associate, Shell technology center, Bangalore, India
9 years ago

For HPLC Reverse phase; wash with unbuffered mobile phase wash with100% Water wash with Methaol or ACN wash with THF or IPA wash with Methylene chloride wash with N-Hepta ... See More

 

Answer added by  Bishoy Ashraf, Quality control senior, Global NAPI Pharmaceutical
9 years ago

regeneration done by organic solvent like acetonitril

 

Answer added by  Ashraf Mohamed, Senior Laboratory Chemist, Arab Company for Laboratories and soil
9 years ago

For Ion Chromatographic columns, you can regenerate your column by one the following methods:- 1. Hydrophilic contamination of low valence: Concentrated eluent of10 times ... See More

 

Answer added by  Wessam AbuShousha, Analyst (Chemist), Pharco Pharmaceuticals
10 years ago

For HPLC columns; Flush it with water in the reverse direction at a flow of1ml/min. for about30 minutes, then flow Isopropanol, Methylene Chloride, Hexane, Methylene Chlo ... See More