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Syed Ashraf's image
Question added by Syed Ashraf Chemist Merck Sharp & Dohem
10 years ago
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Question added by ali alshekh مدير التعليم الطبي المستمر مركز النور التخصصي
10 years ago
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Aavian Hapanyengwi's image  
Answer added by  Aavian Hapanyengwi, qc chemist, Kiarah Chemicals CC
7 years ago

You regenerate HPLC columns by flushing  it with the following volumes of solvent. 20 column volumes of water/acetonitrile (95:5 v/v) 20 column volumes of acetonitrile 5 ... See More

Zisan  Ahamad's image  
Answer added by  Zisan Ahamad, Project Trainee, CSIR-CDRI
7 years ago

by multiple washing with mobile phase they can be regenerate

Faraz ASKARI ABIDI's image  
Answer added by  Faraz ASKARI ABIDI, Quality control officer, Pfizer Pakistan
7 years ago

HPLC columns is regenerate by washing washing with purified water and preserve with methanol or Acetonytrle 50% depend open the type of mobile phase we are using

Virendra Vikhare's image  
Answer added by  Virendra Vikhare, chemist, Rezilabs for Industrial Services / SAECO
7 years ago

To clean and regenerate a reversed phase column flush it with the following volumes of solvent.  water/acetonitrile (95:5 v/v)  acetonitrile  isopropanol hexane  isopropa ... See More

ABDULRAHIM MAHEBOOB MOMIN MOMIN's image  
Answer added by  ABDULRAHIM MAHEBOOB MOMIN MOMIN, SR OFFICER, WOCKHARDT BIOTECH PARK
8 years ago

BY PASSING 1 M NACL SOLUTION THROUGH CHROMATOGRAPHY COLUMN.

KRISHNAMOORTHI THANGAVEL's image  
Answer added by  KRISHNAMOORTHI THANGAVEL, Research Associate, Shell technology center, Bangalore, India
9 years ago

For HPLC Reverse phase; wash with unbuffered mobile phase wash with100% Water wash with Methaol or ACN wash with THF or IPA wash with Methylene chloride wash with N-Hepta ... See More

Bishoy Ashraf's image  
Answer added by  Bishoy Ashraf, Quality control senior, Global NAPI Pharmaceutical
9 years ago

regeneration done by organic solvent like acetonitril

Ashraf Mohamed's image  
Answer added by  Ashraf Mohamed, Senior Laboratory Chemist, Arab Company for Laboratories and soil
9 years ago

For Ion Chromatographic columns, you can regenerate your column by one the following methods:- 1. Hydrophilic contamination of low valence: Concentrated eluent of10 times ... See More