Trending Protein Purification Discussions

Follow

Ask the Community


Ask any professional question and get answers from other specialists.

Stream language
Bilal Alkhizzi's image
Question added by Bilal Alkhizzi Administrative Manager Itqan Medical Center
8 years ago
Answers:
32
Followers:
Views:
418
Vote Count:
0
Answer should contain a minimum of 25 characters.
Aadil Mustafaa Dawud's image  
Answer added by  Aadil Mustafaa Dawud, Senior Analytical Scientist, MEDPHARM Ltd
8 years ago

I would use a reverse phase column, i.e. a column with some sort of organic stationary phase e.g. stationary phase containing methyl group coated or bonded onto the main ... See More

jafar alzoubi's image  
Answer added by  jafar alzoubi
8 years ago

Reversed phase HPLC, where the column material is hydrophopic. The amino acids are eluted by a gradiend of increasing amounts of an organic solvents, such as acetonitrile ... See More

Rajendra Jain Rajendra Jain's image  
Answer added by  Rajendra Jain Rajendra Jain, Quality Manager/ Supervisor/ Sr. Officer, Amnael Pharma
8 years ago

SUPELCOSIL . Particles size 3 microns As well as eclipse xDB-C18

Faraz ASKARI ABIDI's image  
Answer added by  Faraz ASKARI ABIDI, Quality control officer, Pfizer Pakistan
7 years ago

Use TLC/ Column is best method to separate bulk amino acid.  and exactly you want to do means now HPLC - C2 column and oversize also available for separation,

Yusuf Maaeni's image  
Answer added by  Yusuf Maaeni, Office of Agriculture Research /Poultry Research Station /Abu Ghraib /Iraq, Ministry of Agriculture
8 years ago

For a separated, identify, and quantify each component in a mixture. Is being used technique in analytical chemistry, which is high-performance liquid chromatography (H ... See More

Default profile image  
Answer added by  munawar hussain abdul hamid , Good, Pepsi Pakistan
6 years ago

 C18 column could be used for separation of amino acids, but the resolution and the TF (Tailing Factor), FF (fronting factor) and ... of the peaks strongly depends on cho ... See More

Default profile image  
Answer added by  Husein Yehya , Market Access Intern, Janssen Pharmaceutical Company Of Johnson And Johnson
6 years ago

Reversed Phase HPLC could be used, the column is of organic stationary phase, the elution is by organic solvents of increased gradient, therefore separate elutions should ... See More