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Actually you need qPCR Ct values (raw data) for the following: the housekeeping gene: control and experimental conditions the gene being tested: control and experimental ... See More
There are two ways: the delta delta Ct method and the standard curve method (relative or absolute standard curve). Accordingly, the analysis will give the relative values ... See More
by using this formula 2-ΔΔCT method (134). ΔCT = CT (Target gene) – CT (housekeeping gene) ΔΔCT = ΔCT (Sample) – ΔCT (control) 2-ΔΔCT = No. of relative folds
There are many guided informations available in internet, how to set up your Reaction and analyse the data. In a nut shell, once you have your ct curve graph you need to ... See More
The values of Fluorescence in real time PCR emitted by specific probes and by the specific control probe in amplification reactions should be analyzed by the instrument s ... See More
RT-PCR results can be analyzed on the basis of CT value. The CT value depends on instrument’s CT value, Kit standards. It gives the relative fold difference in mRNA expre ... See More
We can use the Ct values which is correlated to the instrument's recommended Ct value.
RT-PCR results analysis based on CT value. CT value depends on instruments setting, Kit standards. Calculation of the expression level or excist of such gene or mutation ... See More
Use the delta delta Ct method to calculate relative fold difference in mRNA expression. use Actin or GAPDH as internal control.