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Very good question. Oxygen absorbs below nm and will interfere with the measurement if carried out in the air. Therefore, for detection of molecules that absorb below nm, ... See More
Based on Beer lambert s law for the relationship between absorbance and the substrate concentration for quantitative analysis
UV visible spectroscopy owrking based on The Beer-Lambert law is the linear relationship between absorbance and concentration of an absorbing species.
Absorption of UV or visible light. Conjugated systems like a benzene ring absorb UV light efficiently
The basic principle of UV visivle spectroscopy is that the absorption of light of a solution is dirrectly proportional to the concentration of that solution.
The amount of light absorbed by a solution is directly proportional to the concentration of the solution.
The Basic Principleof UV-Vis Spectrophotometer is based on the beer's Lambert low. In which a beam of light is passed through the solution absorb the monochromatic r ... See More