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What is the partition chromatography?

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Question ajoutée par Samar Saleh , Community Manager , Bayt.com
Date de publication: 2013/06/23
Bilal Alkhizzi
par Bilal Alkhizzi , Administrative Manager , Itqan Medical Center

- So this chromatographic technique is the first kind of HPLC ever used.

 

- It uses the solubility of the analytes (molecules to be separated) in two immiscible (non-mixing) liquids. One of the liquids is used as the eluant, the other is used as the stationary phase.

 

- The analytes partition, or move back and forth, between the eluant and the stationary phase at certain rates, depending on the characteristics of each analyte.

 

- Therefore, certain analytes will elute first because they solvate in the eluant at a faster rate, while other analytes will elute last, due to their higher solubility in the stationary phase.

KRISHNAMOORTHI THANGAVEL
par KRISHNAMOORTHI THANGAVEL , Research Associate , Shell technology center, Bangalore, India

Partition Chromatography mean by " solute mixing with mobile phase and moving through the stationary phase at the time the solute partition between mobile phase and stationary phase. It is called Normal Phase chromatography.

 

Utilisateur supprimé
par Utilisateur supprimé

This form of chromatography is based on a thin film formed on the surface of a solid support by a liquid stationary phase.
Solute equilibrates between the mobile phase and the stationary liquid.

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