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Delay in emergence of the peaks in chromatogram.
Some times a better separation
Spreading of the Peaks and increasing Tailing Factor (TF) and Reducing the resolution of the peaks
But, in practice, it hardly depend on the analyte, the method of analysis, the nature of mobile phases, and the type of the LC Column
better resolution and longer run time.
1. inrease in resolution
2. improved efficiency
3. increase in retention time.
increase number of theortical plate so better separetion
Increase RT
Better reselution
Better seperation
1- Better seperation between peaks.
2- Better resolution.
3- Increase retainion time which leads to increasing pnjection time, causing more time, and mobile phase to be consumed.
4- Lead to increase pressure due to force needed for flowing mobile phase through the column, where The Pressure (P) = Force (F) / Area (A)
retention time increases
better seperation better resolution
Increase retention time and analysis time compared to a shorter column.
1) better resolution
2) longer R.T
3) Better efficiency
lating appearance of componenet(increase Retention time)