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Increase in HPLC Column length results in?

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Question ajoutée par Ankita Gambhir , Lab Specialist , Scientific Diamond Company
Date de publication: 2017/02/14
Rico Rey Jr Bulan
par Rico Rey Jr Bulan , Quality Control , Munah Food Stuff Factory

  1. Better resolution between peaks - the length of the columns allows for the individual analytes to separate from each other better than if a shorter column were used
  2. Increased chromatographic efficiency (N)- chromatographic efficiency is denoted by the column length (L) divided by the theoretical plate height (H). 
  3. Practicality-wise, longer analytical runs, i.e. longer waiting periods to obtain peaks

Seyyed Amir Siadati
par Seyyed Amir Siadati , Iran , Tofigh Daru Research & Engineering Pharmaceutical Company

Delay in emergence of the peaks in chromatogram.

Some times a better separation

Spreading of the Peaks and increasing Tailing Factor (TF) and Reducing the resolution of the peaks

But, in practice, it hardly depend on the analyte, the method of analysis, the nature of mobile phases, and the type of the LC Column

Jithin Cr
par Jithin Cr , Executive , HOD chemistry , GEOCHEM Laboratories PVT LTD.

better resolution and longer run time.

Aavian Hapanyengwi
par Aavian Hapanyengwi , qc chemist , Kiarah Chemicals CC

1. inrease in resolution

2. improved efficiency

3. increase in retention time.

 

Zisan  Ahamad
par Zisan Ahamad , Project Trainee , CSIR-CDRI

increase number of theortical plate so better separetion

Utilisateur supprimé
par Utilisateur supprimé

Increase RT

Better reselution

Better seperation

 

 

Loay Hassan
par Loay Hassan , QC Methodology supervisor , ACDIMA INT. Trading

1- Better seperation between peaks.

2- Better resolution.

3- Increase retainion time which leads to increasing pnjection time, causing more time, and mobile phase to be consumed.

4- Lead to increase pressure due to force needed for flowing mobile phase through the column, where The Pressure (P) = Force (F) / Area (A)

mehwish hameed
par mehwish hameed , lab instructor , university

retention time increases

better seperation  better resolution

Increase retention time and analysis time compared to a shorter column.

Shibla Malik
par Shibla Malik , Senior Officer , Abbott India Ltd

1) better resolution

2) longer R.T

3) Better efficiency

زكريا عاطف الطباخ
par زكريا عاطف الطباخ , QC supervisor , Pharma Cure for pharmaceutical industries

lating appearance of componenet(increase Retention time)