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Select a layer and press:
0 for100%
1 to9 for decimals from10 to90.
You can also type numbers in fast sequence to set specific opacity such as25,37 or00 for0%.
Pressing ⇧ plus the numbers changes the Fill opacity.
Make sure that you have the Move Tool (V) selected otherwise this will not work.
With the Brush Tool the same shortcuts will set the opacity of the brush.
And in case you don’t know, this is the difference between Opacity and Fill.
Opacity changes the opacity of the entire layer including its styles, whereas Fill keeps the styles intact but knocks out the fill.
5 will make a layer's opacity50% Shift +5 will make a layer's fill50%. -but- 5 will make a layer's opacity50% Shift +5 will ALSO make a layer group's opacity50%
