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Extract RNA Quantify its value (upto1 µg:1 ng-1 µg total RNA or pg- ng poly (A)-RNA) Put on ice immediately after incubation Add dNTPs (mM), nuclease-free water and the t ... See More
on utilise des kittes d'extraction et de purification d'ADN afin d'ameleorer la technique d'amplification par PCR et ainsi southen blott
In RNA sample (up to 1 µg :1 ng-1 µg total RNA or 50 pg-100 ng poly(A)-RNA Add primer oligo d(T) or Random Primer(octamer or decamer). Incubate 5 min at 65 (for oligo dT ... See More
steps for cDNA synthesis: 1- isolate RNA 2- quantify its value for further reaction 3- prepare a reaction containing; oligo dT, water, RNA ample. 4- give it a heat shoc ... See More
this are foods produced from organisms that have had changes introduced into their DNA using the methods of genetic engineering.
genetically engineered foods are foods which have foreign genes inserted in their genetic codes to produce desired traits
By getting the random hexamer or oligo dt primers synthesized and setting up the PCR reaction using reverse transcriptase enzyme.