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1. Conventional PCR gives result only at the end of the reaction while Real Time PCR displays the data of amplification during the run after each cycle. 2. Real Time PCR ... Voir Plus
With a Real time PCR, Detection and Amplification are done on the same instrument.
Actually you need qPCR Ct values (raw data) for the following: the housekeeping gene: control and experimental conditions the gene being tested: control and experimental ... Voir Plus
1. Conventional PCR is more time consuming as it uses gel electrophoresis to analyze the amplified PCR products. In contrast, real-time PCR is less time consuming as ... Voir Plus
There are two ways: the delta delta Ct method and the standard curve method (relative or absolute standard curve). Accordingly, the analysis will give the relative values ... Voir Plus