Trending Recombinant DNA technology Discussions

There is a method to clone a gene without the use of restriction enzymes. Anyone here knows the strategy? It's a primitive technique (hint)!‎

Stem cell research. Do the benefits outweigh the ethical implications? What is your view?‎

How we can determine the ladder we can use in DNA identification? ‎

What do you think about DNA diet tests?‎

 

Answer added by  Noha El Badawy, باحث و نائب مدير المعمل المركزى للتقنيات الحيوية, وزارة الزراعة المصرية_ مركز البحوث الزراعية_ المعمل المركزى للتقنيات الحيوية
10 years ago

according to the expected size of the fragment that we will obtain and the total size of the organism's genome it' ranges from100bp to1 kbp 

 

Answer added by  PBS Kumar P, Clinical Resource Consultant, Becton Dickinson
9 years ago

what range you are expecting will  depends on  your sample.  I guess min50bp to max10kb range of markers available.

 

Answer added by  Haifa Al qudaihy, Deputy Manager PGD Laboratory , Saad Specialist Hospital
8 years ago

There are different methods like: Toposiomerase technology,Sequence and ligase independent cloning, 

 

Answer added by  Marouane BNITIR
8 years ago

pour cloner un gêne sans utilise des enzymes de restriction , on peut se référer a une méthode  simple , nommé la technologie des topoisomerases ,  :          La technolo ... See More

 

Answer added by  Deleted user
8 years ago

Gene can be cloned using PCR (polymerase chain reaction) technique. In this technique copies of fragmented DNA is made using PCR. Those fragmented DNAs are used for makin ... See More

 

Answer added by  Mohammed Jaseem, Academics Support Specialist - Preceptor, Biotecnika
8 years ago

Well - Ethical implications comes into the picture when one carries out research using embryo's (specifically embryonic stem cells). For this kind of research aborte ... See More